Device

Part:BBa_K323149:Design

Designed by: Jernej Turn�ek   Group: iGEM10_Slovenia   (2010-10-20)

CMV ATG cCFP link HIVC His stop term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1026
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct carries BioBrick suffix before the terminator site because a gene was cloned into CMV + pSB1AK8 with XbaI, PstI restriction. Scar is added since BioBricks cannot have restiction sites included. The part was submitted as originally constructed and not in pSB1C3 vector.

Source

References